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recombinant mouse gal 1  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse gal 1
    Recombinant Mouse Gal 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+gal+1/Recombinant+Mouse+Galectin-1+Protein/pm39251592-455-99-104
    Average 92 stars, based on 17 article reviews
    recombinant mouse gal 1 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    In Vitro:

    Article Title: Galectin‐1 deletion in mice causes bone loss via impaired osteogenic differentiation potential of <scp>BMSCs</scp>
    Article Snippet: © 2022 Federation of American Societies for Experimental Biology.. Yu Chen and Yanbin Liu contributed equally to this work.. Abbreviations: BMSCs, bone marrow stromal cells; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; Bsp, bone sialoprotein; Opn, osteopontin; Ocn, osteocalcin; Gal-1, galectin-1; Micro-CT, micro computed tomography; CTX-1, C-terminal telopeptides type I collagen; P1NP, propeptide of type I procollagen; BV/TV, bone volume/total volume; Tb.N, trabecular bone number; Tb.Th, trabecular bone thickness; Tb.Sp, trabecular bone space; Ct.ar/Tt.ar, cortical bone area/total area; Ct.Th, cortical bone thickness; BF, bone formation rate; Dex, dexamethasone; WT, wild type; TRE, TCF Response Element; ChIP, chromatin immunoprecipitatio.

    Recombinant:

    Article Title: Galectin‐1 deletion in mice causes bone loss via impaired osteogenic differentiation potential of <scp>BMSCs</scp>
    Article Snippet: © 2022 Federation of American Societies for Experimental Biology.. Yu Chen and Yanbin Liu contributed equally to this work.. Abbreviations: BMSCs, bone marrow stromal cells; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; Bsp, bone sialoprotein; Opn, osteopontin; Ocn, osteocalcin; Gal-1, galectin-1; Micro-CT, micro computed tomography; CTX-1, C-terminal telopeptides type I collagen; P1NP, propeptide of type I procollagen; BV/TV, bone volume/total volume; Tb.N, trabecular bone number; Tb.Th, trabecular bone thickness; Tb.Sp, trabecular bone space; Ct.ar/Tt.ar, cortical bone area/total area; Ct.Th, cortical bone thickness; BF, bone formation rate; Dex, dexamethasone; WT, wild type; TRE, TCF Response Element; ChIP, chromatin immunoprecipitatio.

    Article Title: Modulation of galectin-9 mediated responses in monocytes and T-cells by pregnancy-specific glycoprotein 1
    Article Snippet: Recombinant human GAL-9 was purchased from different suppliers including R&D systems (catalog# 2045-GA and 9064-GA), BioLegend (catalog# 557306), and MyBioSource(catalog# MBS2097223). .. Recombinant mouse GAL-1 (catalog# 1245-GA/CF) and GAL-9 (catalog# 3535-GA/CF) were purchased from R&D systems and mouse GAL-3 (catalog# ABIN 7274752) and mouse GAL-1 with an Fc tag (catalog# ABIN7274695) were purchased from antibodies-online.com . .. Recombinant human Tim-3-Fc was purchased from BioLegend (catalog# 759904).

    Article Title: The Role of Galectin-1 and Galectin-3 in the Mucosal Immune Response to Citrobacter rodentium Infection
    Article Snippet: For migration assays, a 24 well transwell plate containing 8.0 μm pore membrane inserts (Appleton Woods, Birmingham, UK) was used. .. Top wells contained 5×10 4 J774 cells in 100 μl serum-free DMEM while bottom wells contained 600 μl serum free DMEM plus 100 ng/ml or 1 μg/ml recombinant mouse Gal-1 (R&D Systems, Abingdon, UK). .. Recombinant mouse monocyte chemoattractant protein-1 (MCP-1) (R&D Systems, Abingdon, UK) at 100 ng/ml was used as a positive control and media alone as a negative control.

    Article Title: GDNF promotes neurite outgrowth and upregulates galectin-1 through the RET/PI3K signaling in cultured adult rat dorsal root ganglion neurons.
    Article Snippet: Galectin-1 (GAL-1), a member of a family of b-galactoside binding animal lectins, is predominantly expressed in isolectin B4 (IB4)-binding small non-peptidergic (glial cell line-derived neurotrophic factor (GDNF)-responsive) sensory neurons in the sections of adult rat dorsal root ganglia (DRG), but its functional role and the regulatory mechanisms of its expression in the peripheral nervous system remain unclear.. In the present study, both recombinant nerve growth factor (NGF) and GDNF (50 ng/ml) promoted neurite outgrowth from cultured adult rat DRG neurons, whereas GDNF, but not NGF, significantly increased the number of IB4-binding neurons and the relative protein expression of GAL-1 in the neuronenriched culture of DRG.. The GAL-1 expression in immortalized adult rat Schwann cells IFRS1 and DRG neuron-IFRS1 cocultures was unaltered by treatment with GDNF, which suggests that GDNF/GAL-1 signaling axis is more related to neurite outgrowth, rather than neuron-Schwann cell interactions.

    Article Title: Modulation of galectin-9 mediated responses in monocytes and T-cells by pregnancy-specific glycoprotein 1.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Tumor galectin-1 mediates tumor growth and metastasis through regulation of T-cell apoptosis
    Article Snippet: .. T-cells were divided into 4 treatment groups: control, recombinant mouse Gal-1 (rmGal-1:10 μg/ml, R&D Systems, Minneapolis, MN), ConA, and ConA + rmGal-1. ..

    Article Title: Local administration of regulatory T cells promotes tissue healing.
    Article Snippet: .. Primary monocytes were isolated using the EasySep Mouse Monocyte Isolation Kit (STEMCELL Technologies), according to the manufacturer’s instructions, and cultured in DMEM/F12 (Thermo Fisher Scientific) with 10% FBS, 10 ng/ml M-CSF (PeproTech) and 100 units/ml penicillin/streptomycin, for 3 days, with or without 0.1 ng/ml IFN-γ (STEMCELL Technologies, #78021), and/or 1 ng/ml IL-1β (PeproTech, #211-11B), in the presence of one of the Treg-derived factors: recombinant mouse IL-10 (10 ng/ml, PeproTech, #210-10-10), recombinant human ANXA1 (500 ng/ml, R&D systems, #3770-AN-050), recombinant human ANXA2 (500ng/ml, R&D systems, #9409-AN-050), recombinant mouse FGL-2 (375 ng/ml, R&D systems, #5257-FL), recombinant mouse Areg (500ng/ml, PeproTech, #315-36), recombinant mouse Gal-1 (500 ng/ml, R&D Systems, #1245-GA), or recombinant mouse Gal-3 (500ng/ml, R&D Systems, #1197-GA). .. Monocytes were cultured at 37 °C with 5% CO2 for 3 days, while sorted Mo/MΦ were cultured for 48 h before detaching them with TrypLE (Thermo Fisher Scientific) containing 2mM EDTA and resuspending them in PBS for subsequent staining steps.

    Control:

    Article Title: Tumor galectin-1 mediates tumor growth and metastasis through regulation of T-cell apoptosis
    Article Snippet: .. T-cells were divided into 4 treatment groups: control, recombinant mouse Gal-1 (rmGal-1:10 μg/ml, R&D Systems, Minneapolis, MN), ConA, and ConA + rmGal-1. ..

    Isolation:

    Article Title: Local administration of regulatory T cells promotes tissue healing.
    Article Snippet: .. Primary monocytes were isolated using the EasySep Mouse Monocyte Isolation Kit (STEMCELL Technologies), according to the manufacturer’s instructions, and cultured in DMEM/F12 (Thermo Fisher Scientific) with 10% FBS, 10 ng/ml M-CSF (PeproTech) and 100 units/ml penicillin/streptomycin, for 3 days, with or without 0.1 ng/ml IFN-γ (STEMCELL Technologies, #78021), and/or 1 ng/ml IL-1β (PeproTech, #211-11B), in the presence of one of the Treg-derived factors: recombinant mouse IL-10 (10 ng/ml, PeproTech, #210-10-10), recombinant human ANXA1 (500 ng/ml, R&D systems, #3770-AN-050), recombinant human ANXA2 (500ng/ml, R&D systems, #9409-AN-050), recombinant mouse FGL-2 (375 ng/ml, R&D systems, #5257-FL), recombinant mouse Areg (500ng/ml, PeproTech, #315-36), recombinant mouse Gal-1 (500 ng/ml, R&D Systems, #1245-GA), or recombinant mouse Gal-3 (500ng/ml, R&D Systems, #1197-GA). .. Monocytes were cultured at 37 °C with 5% CO2 for 3 days, while sorted Mo/MΦ were cultured for 48 h before detaching them with TrypLE (Thermo Fisher Scientific) containing 2mM EDTA and resuspending them in PBS for subsequent staining steps.

    Cell Culture:

    Article Title: Local administration of regulatory T cells promotes tissue healing.
    Article Snippet: .. Primary monocytes were isolated using the EasySep Mouse Monocyte Isolation Kit (STEMCELL Technologies), according to the manufacturer’s instructions, and cultured in DMEM/F12 (Thermo Fisher Scientific) with 10% FBS, 10 ng/ml M-CSF (PeproTech) and 100 units/ml penicillin/streptomycin, for 3 days, with or without 0.1 ng/ml IFN-γ (STEMCELL Technologies, #78021), and/or 1 ng/ml IL-1β (PeproTech, #211-11B), in the presence of one of the Treg-derived factors: recombinant mouse IL-10 (10 ng/ml, PeproTech, #210-10-10), recombinant human ANXA1 (500 ng/ml, R&D systems, #3770-AN-050), recombinant human ANXA2 (500ng/ml, R&D systems, #9409-AN-050), recombinant mouse FGL-2 (375 ng/ml, R&D systems, #5257-FL), recombinant mouse Areg (500ng/ml, PeproTech, #315-36), recombinant mouse Gal-1 (500 ng/ml, R&D Systems, #1245-GA), or recombinant mouse Gal-3 (500ng/ml, R&D Systems, #1197-GA). .. Monocytes were cultured at 37 °C with 5% CO2 for 3 days, while sorted Mo/MΦ were cultured for 48 h before detaching them with TrypLE (Thermo Fisher Scientific) containing 2mM EDTA and resuspending them in PBS for subsequent staining steps.

    other:

    Article Title: Local administration of regulatory T cells promotes tissue healing.
    Article Snippet: ELISAs for cytokines in injured muscle Homogenised muscle tissue was incubated for 30min on ice in T-PER Tissue Protein Extraction Reagent (Thermo Fisher Scientific) using 10ml/g of tissue containing 1 tablet of protease inhibitor (Roche)/7ml.



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    Image Search Results


    ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.

    Journal: bioRxiv

    Article Title: Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary Fibroblasts

    doi: 10.64898/2026.04.02.716135

    Figure Lengend Snippet: ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.

    Article Snippet: The working concentration for the following reagents can be found in the text and figure legends: Human IL-1RA Recombinant Protein (IL1Ra) (PeproTech®, 200-01RA), Mouse IL-1 alpha Recombinant Protein, (PeproTech®, 211-11A), Mouse IL-1α Neutralizing Antibody (Invivogen, Anti-mIL-1α-mIgG1 clone 6H7, mIL-1α-mab9-02), Recombinant Mouse IgG1 Isotype Control Antibody (Invivogen, Anti-β-Gal-mIgG1 clone T9C6, bgal-mab9-02), Human TGF-β 1 Recombinant Protein (PeproTech®, 100-21), Human GDF15 Recombinant Protein (PeproTech®, 120-28C), Human VEGF Recombinant Protein (PeproTech®, 450-32), Human OPN Recombinant Protein (PeproTech®, 120-35), Murine CXCL1 Recombinant Protein (PeproTech®, 250-11), Human IGFBP3 Recombinant Protein (PeproTech®, 100-08), Human TNFRSF11B Recombinant Protein (PeproTech®, 450-14), Murine CXCL5 Recombinant Protein (PeproTech®, 250-17), and Muring CCL20 Recombinant Protein (PeproTech®, 250-27).

    Techniques: Enzyme-linked Immunosorbent Assay, Gene Expression, Quantitative RT-PCR, Cell Culture, Titration, Control, Magnetic Beads, Comparison